Rebin, S.; Tulay, P. ; Hazha, J.
Vol. 9, Jan-Jun 2020
Abstract:
Abortion is the most common complication during pregnancy. Given the importance of thrombophilia in pregnant women and it is association with recurrent pregnancy loss. Polymorphism analysis of the genes involved in thrombophilia may be useful. There are many factors that affect abortion and recurrent miscarriage such as Female age and embryonic aneuploidy, Antiphospholipid syndrome, Parental structural chromosomal abnormalities, Uterine structural abnormalities, Endocrine factors: thyroid function, PCOS and prolactin, Immunological factors and progesterone supplementation, TLC, infection, lifestyle and sperm DNA damage and one of the factors is Inherited thrombophilia that include several genes that may have an impact on recurrent miscarriage. We have analyzed seven genes in the Iraq-Erbil population to determine the relationship between these genes and the number of abortions, age and family history. Blood samples were taken from 50 women with recurrent miscarriages and another 30 women who did not have any abortions as control cases and using PCR-RFLP, researchers analyzed and extracted DNA from samples for carrying Methylene Tetrahydrofolate Reductase (MTHFR) C677T and A1298C, Plasminogen Activator Inhibitor-1 (PAI-1), Factor V Leiden, Prothrombin G20210A , Factor XIII and Fibrinogen beta chain polymorphisms. There was no association between recurrent miscarriage with age however the two genes MTHFR C677 and MTHFR A1298C were related to family history. After comparing the genes among themselves, the static analysis was that MTHFR C677T and PAI-1 (4G/5G) are associated with recurrent pregnancy loss. But after comparing the genes with control cases, the static result of the analysis was that all genes are associated with recurrent miscarriage and are significant. However, further investigate on large scale populations perhaps needed to recognize new genetics diverse.