Surabhi Verma, Dr.Shuchi Mitra
Vol. 8, Jul-Dec 2019
Abstract:
GulabArkaRose water is the major auxiliary product which has been produced since ancient time. It is obtained through the distillation process. Due to its fragrance and medicinal value, it is used in cosmetic, food, flavouring, levigation and as anupana. The shelf life of ArkaKalpanais 1 year as perD&C 1940. The major problem during its storage is fungal growth, which diminishes its quality. AIM & OBJECTIVE: To identify the fungus. MATERIAL & METHODS In this study, Rose water was prepared with fresh rose petals procured from local market of Haridwar. DegBhapkaYantra was used for steam distillation process; it was washed properly with hot water then dried in sun light properly for sterilization and using IPA(Isopropyl alcohol). Then it was kept in air tight bottle and stored at normaltemperature. Arkawas prepared under sanitised conditions but it developed an abnormal cottony growthalong with the mild hazing after 4months of manufacturing. For fungal culture Rose Bengal agar solution was used After autoclaving, media was poured onto petri dish and left for drying. After 24hours it was checked for contamination. Then 100microltr of sample was spreaded properly onto fresh plate and kept in incubator. After 72hours fungus was grown.Lectophenol cotton bluestain is used for slide preparation and this slide is observed under microscope. RESULT & CONCLUSION Identification of fungus was done on the basis of morphological.A mass of branching, thread-like hyphae and finger like projection were seen on microscope. On the basis of these characters the fungus was identified as mycelium.