Premalatha Shetty, Vishwanatha
Vol. 6, Jul-Dec 2018
Abstract:
Garcinia, Citrus and Artocarpusspecies are known for their medicinal properties in the folklore especially in alleviation of biliousness. Chloroform and alcoholic extracts of the fruit parts of Garcinia morella(Mor), G mangostana (MS), Citrus maxima (CMx), C aurantium (CA) andArtocarpusgomezianus (AG) were prepared. The extracts and the bile acids namely, Cholic acid (ChA), Deoxycholic acid (DCA), Chenodeoxycholic acid (CDCA) and Lithocholic acid (LCA) were investigated for their effect on Hep G2 liver carcinoma cells and Murine 3T3-L1 fibroblast cell lines by MTT assay. The ability of the fruit extracts at ameliorating bile acid induced toxicity and effect on adipogenesis in differenting 3T3 cells was studied. Alcoholic extract of mangostana rind exhibited 30.6 mg of antioxidant capacity equivalent to ascorbic acid in gram of the dried rind which was 5 to 25 times higher in comparison to the other alcoholic extracts tested as per DPPH assay. Dose dependent effect of the extracts was observed on the cells. With the exception of G mangostana, Hep G2 cells were found to be sensitive to the presence of high concentration of the extracts (extract derived from 6mg and 10mg of the dried fruit part per ml, for alcoholic and chloroform extracts respectively). As per the MTT assay results, the metabolic function of the cells was enhanced at lower concentrations of the extracts. Morella extracts were found to exert toxicity to a significant level on both the cell lines at high concentrations. IC50 concentrations of ChA, DCA, CDCA and LCA for 3T3 were 0.53, 0.21, 0.18 and 0.19mM and Hep G2 was more sensitive as the values were 0.38, 0.14, 0.11 and 0.11 mM respectively. Secondary bile acids LCA and CDCA were more toxic than primary bile acids. Alcoholic extract of MS was effective in protecting against the toxicity induced by DCA, LCA and CDCA in both the cells. AG extract appeared to influence adipogenesis in 3T3 cells.