Abhijeet S Pohekar, Dr Niraj Gupta
Vol. 12, Jul-Dec 2021
Abstract:
The sense of seeing is perhaps the most crucial. More than 80% of the information we get from the outside world comes to us through visual sources. The main aim of the study is to cultivation of limbal stem cells using amniotic membrane, both intact and denuded. The methodology section describes the steps taken to prepare, preserve, and use human amniotic membrane for the cultivation of limbal stem cells. It is concluded that the as a result, when limb epithelial cells are cultivated on an intact AM, the basal layer of the cultured cells express ABCG2, whereas Connexin-43 and keratins 3 and 12 are expressed at modest levels.
DOI: http://doi.org/10.37648/ijrmst.v11i02.023